Evaluation of antibacterial activity of gum-resin extracts of Boswellia serrata against human pathogens

Altun M., Karakaş İ.

4th International Eurasian Conference on Science, Engineering and Technology (EurasianSciEnTech 2022), Ankara, Turkey, 14 - 16 December 2022, pp.275-279

  • Publication Type: Conference Paper / Full Text
  • City: Ankara
  • Country: Turkey
  • Page Numbers: pp.275-279
  • Çanakkale Onsekiz Mart University Affiliated: Yes


Extensive use of synthetic antibiotics can lead to chemical side effects and bacterial resistance. Medicinal plants containing bioactive components have gained importance due to their wide availability, fewer complications, and low cost for treating bacterial infections. This study aims to determine the antibacterial activity of gum-resin hydroalcoholic extract of Boswellia serrata (B.serrata) on bacterial strains. The hydroalcoholic extract was obtained by maceration technique waiting for 72 h. The antimicrobial activity of B.serrata resin extract at four different concentrations (100, 50, 25, 12.5 mg/ml) against Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29212, Proteus vulgaris ATCC 13315, Bacillus subtilis ATCC 6633, Streptococcus agalactiae ATCC 12386, Pseudomonas aeruginosa ATCC 10145, Streptococcus epidermidis ATCC 12228, Streptococcus pyogenes ATCC 19615, and Staphylococcus aureus ATCC 25923 bacterial strains was detected using disc diffusion and microdilution assay. Gentamicin, ampicillin and vancomycin were used as positive controls. Dimethyl Sulfoxide (DMSO) was used as a negative control. The percentage yield of extract was found to be 63.84 %. Compared with standard antibiotics, while the extract of gum resin showed a high zone of inhibition against B.subtilis (16±0.816 mm), S.aureus (13±0.816 mm), E.faecalis (13.25±0.50 mm), and P.vulgaris (13 mm) strains; the extract exhibited no appreciable antibacterial activity against S.agalactiae (9±0.816), P.aeruginosa (8.75±0.50 mm), S.pyogenes (9.25±0.50 mm), S.epidermidis (10.75±0.957 mm), and E.coli (11.66±2.39 mm) at a concentration of 100 mg/ml. Minimum inhibitory concentration (MIC) results varied between 12.5 mg/ml and 100 mg/ml, and the highest MIC value was obtained from the B.subtilis strain. The extract obtained from the gum resin of B.serrata may be used for the treatment of bacterial diseases as a remedial agent. The investigations should be done on the determination of chemical characterization and cytotoxicity of plant extracts for clinical use. Keywords: Boswellia serrata, antibacterial activity, gum resin, disc diffusion assay