7th International Scientific Agriculture Symposium “Agrosym 2016”, Jahorina, Bosnia And Herzegovina, 6 October - 09 December 2016, vol.1, no.1, pp.562-567
ne"> There are numerous factors affecting pollen germination in plants. Plant growth regulators have produced different results on pollen germination both in vivo and in vitro. In this research, pollen viability using IKI test and effects of three different growth regulators (epibrassinolide, homobrassinolide and gibberellic acid) added into growth medium on the in vitro pollen germination of two wine grape cultivars (Cabernet Sauvignon and Merlot) were investigated. Pollen viability results showed that Cabernet Sauvignon contained more viable pollens (63.12% that Merlot (59.06%). Pollens collected from fresh flowers were sown on medium containing 1% agar and 20% sucrose using ‘agar in petri dish’ method. Results revealed that growth regulators did not affect germination in the cultivars, but the treatments had an overall increasing influence (about 25%). Although, in general, treatments produced similar ratios with the control group, 100 mgL-1 GA3, alone, and in combination with 0.01 and 0.001 mgL-1 epibrassinolide were found to cause significant increase. In addition, applying the lowest GA3 concentration (25 mgL-1) with 0.1 mgL-1 epibrassinolide provided the highest germination (23.54%). Only 0.001 mgL-1 homobrassinolide resulted in the almost the same ratio with the control group. Information obtained here show that effects of growth regulators change with the genotype, type of the hormones and composition of the basal growth medium.