Research Information System
8. International Symposium on Ecology and Environmental Problems (ISEEP)., Çanakkale, Turkey, 4 - 07 October 2017, vol.1, no.1, pp.1
Knowing the yeast population on grape berries and musts has importance for wine fermentations, since different yeast species, genera and strains with their metabolic activity effect quality and property of wine. There is a variety of moleculer methods for yeast identification. Recently, polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) of ITS-5.8S rDNA gene and sequence analysis of rDNA gene region have utilized for this objective. These techniques have proved to be useful for the rapid identification of yeast species. The aim of this study was to investigate restriction profiles of yeast strains isolated from Kalabaki wine grapes in Gökçeada. For this purpose, grape samples were collected from previously determined vineyard. Samples were homogenized, diluted then inoculated on YGC solid medium and incubated 2-3 days at 30 ºC. Growing yeast strains were inoculated on YPD solid medium and incubated 2-3 days at 30 ºC. Genomic DNA extraction of yeast strains was done and ITS1-5.8S-ITS2 rDNA gene regions were amplified by using ITS1 and ITS4 primers. PCR amplicons were digested by using 5 restriction enzymes ( HinfI, HaeIII, MspI, AluI and HhaI ). As a result, we have analysed 34 yeast strains that are isolated from Kalabaki wine grapes. 3 groups were obtained by length of PCR product. According to RFLP results 8 groups and 8 different profiles were attained. This is the first study for definition of restriction profiles of yeast strains with regard to Gökçeada Kalabaki Wine Grapes.