Genotoxic effects of Rhamnus catharticus in huma blood lymphocyte

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Çiçekliyurt M. M., Akkuş G., Yüksel Balım D.

Acta Oecologica Carpatica, no.XI.II, pp.91-98, 2018 (Peer-Reviewed Journal)

  • Publication Type: Article / Article
  • Publication Date: 2018
  • Journal Name: Acta Oecologica Carpatica
  • Journal Indexes: Art Index
  • Page Numbers: pp.91-98
  • Çanakkale Onsekiz Mart University Affiliated: Yes


Rhamnus catharticus L., has an antioxidant and antimicrobial activity whereas the cytotoxic effect of the plant may be responsible for the emergence of these properties. The aim of this study was to evaluate the genotoxic and cytotoxic potential of the water and methanolic extract obtained from the Rhamnus catharticus leaf in peripheral lymphocyte culture by micronucleus assay for 24- and 48-hours treatment period. Three doses of Rhamnus catharticus extract (1, 2, 4 mg/ml) were selected as treatment group; Mitomycin C (MMC) as positive and water is used as negative control. We found that all extracts had greater toxicity compared to the negative control and MMC toxicity was higher than other concentrations. This might be caused by the various ingredients from the plant extract that are obtained due to the polarity of solvents.