Introduction: The aim of this study was to compare the cyclic fatigue resistances of nickel-titanium rotary glide path files, which are manufactured as single-file systems, under dynamic model. Methods: Twenty Pro Glider (16.02) and 20 One G (14.03) nickel-titanium glide path files were included in this study. Cyclic fatigue tests were performed with specifically manufactured dynamic cyclic testing device. The device has an artificially prepared canal with 60 curvature angle and 5-mm curvature radius. The canal inner diameter is 1.5 mm, and its curvature center is located at the corona! 5 mm from the apical. The files were randomly divided into 2 groups (n = 20): group 1, ProGlider; group 2, One G. Time to fracture was recorded, and the number of cycles to failure for each instrument was obtained. Statistical analyses were performed by using independent samples t test. The statistically significant level was set at P < .05. Results: The cyclic fatigue resistance of Pro Glider files was found to be statistically significantly higher than that of the One G files (P < .05). Conclusions: Within the limitations of the present study, it was concluded that the cyclic fatigue resistance of Pro Glider, which is manufactured as single-file glide path system, is higher than that of the One G file.