The Role of SWI/SNF Chromatin Remodeling Complex in the Metabolism Of Trehalose


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Turgut Genç T. , Akıncı N.

International Journal of Recent Scientific Research, cilt.9, ss.29024-29029, 2018 (Diğer Kurumların Hakemli Dergileri)

  • Cilt numarası: 9 Konu: 9
  • Basım Tarihi: 2018
  • Doi Numarası: 10.24327/ijrsr.2018.0909.2780
  • Dergi Adı: International Journal of Recent Scientific Research
  • Sayfa Sayıları: ss.29024-29029

Özet

Trehalose is required for stress tolerance and regulation of glucose uptake. Trehalose-6-phosphate

synthase enzyme, encoded by TPS1, is the first enzyme in trehalose biosynthetic pathway. The

neutral trehalase enzyme, encoded by NTH1 gene, is responsible from the hydrolysis of stress

accumulated trehalose in Saccharomyces cerevisiae. It is known that promoter regions of

transcriptionally active genes are cleared by chromatin modifying complexes to provide access to

transcription initiation complex. In this study, we have analyzed if the chromatin modifying

complexes involves in the transcription of NTH1 and TPS1 genes under normal and stress inducing

growth conditions. We tested the effect of Snf2p, catalytic subunit of SWI/SNF complex, on the

transcriptional regulation of NTH1 and TPS1. We have found that NTH1 gene expressions in Δsnf2

mutants were 3-fold higher than wild type yeast strain, but TPS1 gene expression in Δsnf2 mutants

didn’t differ from wild type yeast strain. The nitrogen starvation triggered only NTH1 gene

expression in wild type yeast cells but no change was observed in Δsnf2 mutants and TPS1 gene

expression. Our results indicated that SWI/SNF chromatin remodeling complex is essential for the

transcriptional repression of NTH1 gene, but not TPS1 gene.