Research Information System
International Journal of Recent Scientific Research, vol.9, no.9, pp.29024-29029, 2018 (Peer-Reviewed Journal)
Trehalose is required for stress tolerance and regulation of glucose uptake. Trehalose-6-phosphate
synthase enzyme, encoded by TPS1, is the first enzyme in trehalose biosynthetic pathway. The
neutral trehalase enzyme, encoded by NTH1 gene, is responsible from the hydrolysis of stress
accumulated trehalose in Saccharomyces cerevisiae. It is known that promoter regions of
transcriptionally active genes are cleared by chromatin modifying complexes to provide access to
transcription initiation complex. In this study, we have analyzed if the chromatin modifying
complexes involves in the transcription of NTH1 and TPS1 genes under normal and stress inducing
growth conditions. We tested the effect of Snf2p, catalytic subunit of SWI/SNF complex, on the
transcriptional regulation of NTH1 and TPS1. We have found that NTH1 gene expressions in Δsnf2
mutants were 3-fold higher than wild type yeast strain, but TPS1 gene expression in Δsnf2 mutants
didn’t differ from wild type yeast strain. The nitrogen starvation triggered only NTH1 gene
expression in wild type yeast cells but no change was observed in Δsnf2 mutants and TPS1 gene
expression. Our results indicated that SWI/SNF chromatin remodeling complex is essential for the
transcriptional repression of NTH1 gene, but not TPS1 gene.