Royal jelly attenuates gastric mucosal injury in a rat ethanol-induced gastric injury model

DURAN Y., KARABOĞA İ., POLAT F. R., POLAT E., Erboga Z. F., OVALI M. A., ...More

MOLECULAR BIOLOGY REPORTS, vol.47, no.11, pp.8867-8879, 2020 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 47 Issue: 11
  • Publication Date: 2020
  • Doi Number: 10.1007/s11033-020-05939-w
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.8867-8879
  • Keywords: Royal Jelly, Gastric ulcer, Inducible nitric oxide synthase, Nuclear factor-kappa beta, Apoptosis, OXIDATIVE STRESS, IN-VIVO, SUPEROXIDE-DISMUTASE, ULCER, ACID, INHIBITION, INFLAMMATION, ANTIOXIDANT, INVOLVEMENT, ACTIVATION
  • Çanakkale Onsekiz Mart University Affiliated: Yes


The aim of the study was to investigate traditionally used Royal Jelly (RJ) for treating an ethanol-induced gastric ulcer model in rats. A total of 32 Wistar albino male rats were divided into 4 groups of 8: group I = Control, group II = Ethanol, group III = RJ + Ethanol, and group IV = Lansoprazole + Ethanol. In groups II, III, and IV, animals were administered 1 ml of absolute ethanol orally after a 24-h fast to induce ulcer formation. The histopathological changes in the gastric mucosa were determined using hematoxylin-eosin (H&E) staining. Immunohistochemically, inducible nitric oxide (iNOS) and nuclear factor kappa beta (Nf-kappa beta) markings were evaluated in gastric tissue. Cell death in the gastric mucosa was determined by the TUNEL method. Oxidative status markers, superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT), and myeloperoxidase (MPO) levels were determined spectrophotometrically. Expression of the interleukin - 1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha) genes in gastric tissues was determined by real-time PCR; and TNF-alpha, IL-10, and IL-1 beta levels were determined. RJ was found to inhibit iNOS and Nf-kappa beta activity in the gastric mucosa and prevent epithelial cell apoptosis. In particular, pro-inflammatory cytokines TNF-alpha and IL-1 beta levels were significantly decreased in the RJ + Ethanol group compared to the Ethanol group. In addition, a decrease in the MPO level indicated that RJ prevented tissue damage, especially by preventing inflammatory cell infiltration. The study demonstrated a possible gastroprotective effect of RJ in a rat ethanol-induced gastric ulcer model.