Occurrence and Genetic Diversity of Celery Mosaic Virus (CeMV, Potyvirus apiumtessellati) Isolates from Türkiye
JOURNAL OF CROP HEALTH, cilt.78, sa.135, ss.1-12, 2026 (SCI-Expanded)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 78 Sayı: 135
- Basım Tarihi: 2026
- Doi Numarası: 10.1007/s10343-026-01403-0
- Dergi Adı: JOURNAL OF CROP HEALTH
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED)
- Sayfa Sayıları: ss.1-12
- Çanakkale Onsekiz Mart Üniversitesi Adresli: Evet
Özet
Celery mosaic virus (CeMV, Potyvirus apiumtessellati) is an important viral pathogen of celery worldwide; however, information on its occurrence in Türkiye and the genetic diversity of global isolates remains limited. In this study, 217 symptomatic and 18 asymptomatic celery samples were collected during the 2020 growing season from the Aegean and Marmara regions, the major celery-producing areas of Türkiye. Molecular diagnostics confirmed CeMV infection in 109 samples. Genetic diversity and population structure were analyzed using the complete coding sequences of the coat protein (CP), nuclear inclusion b (NIb), and concatenated NIb-CP gene regions. Pairwise sequence comparisons showed that Türkiye isolates shared 96.3–98.1% (CP), 95.6–96.3% (NIb), and 96.2–97.3% (NIb-CP) nucleotide identity with global isolates. Phylogenetic analyses grouped CP, NIb, and NIb-CP sequences into three, four, and four clades, respectively. Türkiye isolates clustered within Clade I based on the CP gene, whereas they were distributed between Clade I and Clade II in NIb and NIb-CP analyses. Genetic diversity analyses revealed high haplotype diversity and moderate nucleotide diversity across all gene regions, with Türkiye isolates exhibiting slightly higher diversity than global populations. Analysis of molecular variance indicated that most genetic variation occurred within populations, while ΦST values revealed low to moderate but significant genetic differentiation among populations. Evidence of negative selection was detected in both genes, with stronger selective pressure acting on the NIb region. Overall, this study provides the first comprehensive insight into the molecular epidemiology and evolutionary dynamics of CeMV.