A molecular assay for quantification and simultaneous detection following multi extraction of DNAs of soybean and poultry in sausages

ŞAKALAR E.

FOOD SCIENCE AND BIOTECHNOLOGY, vol.24, no.6, pp.1915-1920, 2015 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 24 Issue: 6
  • Publication Date: 2015
  • Journal Name: FOOD SCIENCE AND BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1915-1920
  • Çanakkale Onsekiz Mart University Affiliated: Yes

Abstract

A duplex polymerase chain reaction assay was designed for the simultaneous identification of soybean and poultry used in sausages. Multi-Fast DNA Isolation Kit was used for simultaneous extraction of amplifiable soybean and poultry DNA from sausages. The primer generated specific DNA fragments of 173 and 183 bp in length for soybean and poultry, respectively. The duplex assay sensitivity was 0.35% for the specific detection of poultry mixed with soybean and other additive materials, and it was 0.05% for the specific detection of soybean mixed with poultry and other additive materials. The optimized duplex PCR assay was applied to 15 commercial sausages as a quick preliminary examination. The results related to unintentional or intentional contaminants were analyzed by real-time PCR based SQT-DNA (Sakalar Quantification Table of DNA). The techniques developed here are suitable tools for the qualitative and quantitative comparison of sausage content.