Qualitative analysis of meat and meat products by multiplex PCR technique

ŞAKALAR E., ABASIYANIK M.

AFRICAN JOURNAL OF BIOTECHNOLOGY, vol.10, no.46, pp.9376-9386, 2011 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 10 Issue: 46
  • Publication Date: 2011
  • Journal Name: AFRICAN JOURNAL OF BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Aquatic Science & Fisheries Abstracts (ASFA), Biotechnology Research Abstracts, CAB Abstracts, Pollution Abstracts, Veterinary Science Database
  • Page Numbers: pp.9376-9386
  • Çanakkale Onsekiz Mart University Affiliated: No

Abstract

Multiplex polymerase  chain  reaction  (M-PCR)  assay  was  applied  to processed  and  raw  meats  for  the identification of the most used species in foodstuffs such as, ruminant, poultry, fish and pork materials. Specific-species primers, designed according to theconservative regions of 16S rRNA, were used after alignment  of  the  available  sequences  in  the  GenBank database. The  primers  generated  specific  DNA fragments  of  183,  224,  290  and  374  bp  length  for  poultry,  fish,  pork  and  ruminant,  respectively. The optimized M-PCR  assay  was  applied to  93  commercial  meat products  and  it  showed  the  presence of poultry meat in red meat analyzed, although, it was not indicated on the label.