The effect of bacteria-based formulations on tea (Camellia sinensis L.) growth, yield, and enzyme activities

Çakmakçı R., Ertürk Y., Varmazyari A., Atasever A., Kotan R., Haliloğlu K., ...More

Annals of Warsaw University of Life Sciences - SGGW - Horticulture and Landscape Architecture, vol.38, pp.5-18, 2017 (Peer-Reviewed Journal)


There is an increasing need to use microorganisms for safe crop production for consumers, as well as to prevent environmental pollution and ensure the sustainability of agriculture and agricultural resources. The objective of this study was to evaluate possible effects of mineral fertilizer (NPK), one commercial liquid bio-fertilizer and ACC deaminase-contain-ing, N2-fixmg, and P-solubilizing bacteria-based bio-fertilizers in triple strain combinations (BF1: Bacillus subtilis RC28 + Paenibacillus polymyxa RC05 + Pseudomonas fluorescens RC77; BF2: Bacillus subtilis RC63 + Paenibacillus polymyxa 24/3 + Pseudomonas fluorescens 48/3; BF3: Bacillus atrophaeus 36/10 + Paenibacillus polymyxa 28/3 + Pseudomonas fluorescens 51/2; BF4: Bacillus subtilis 39/3 + Bacillus subtilis RC63 + + Pseudomonas fluorescens 53/6; BF5: Bacillus subtilis RC521 + Paenibacillus polymyxa 66/6 + Pseudomonas fluorescens RC77; BF6: Bacillus megaterium 12/1 + Paenibacillus polymyxa RC35 + Pseudomonas fluorescens 48/3) on the growth and enzyme activities in tea under natural acidic conditions over three years. The bio-fertilizer formulations stimulated overall plant growth, including shoot development, plant height, trunk diameter, leaf area, leaf yield, chlorophyll and an-thocyanin content, and activities of oxidative, catalytic, hydrolytic and anti-oxidative enzymes, in the Turkish registered tea clones Tuglah-10. In addition, inoculation with bacterial formulation affected the activities of enzymes such as glutathione reductase, glutathione S-transferase, glucose-6-phosphate dehydrogenase, 6-phospho-gluconate dehydrogenase, polyphenol oxidase, peroxidase, urease, 5-dehydroshikimate reduc-tase, and alcohol dehydrogenases. However, plant growth responses were variable and dependent on the formulations and parameter evaluated. The selected effective bio-formulations could play an important role in understanding the plants' tolerance and adaptation to stress, and may contribute to improving the quality of tea products. Their ability to enhance plant growth will enable reductions in inputs of chemical fertilizer, and they have the potential to be used as a bio-fertilizer in sustainable and organic tea production. Our results indicate that a higher leaf yield potential in tea plants with bacterial inoculation can be expected on acidic soils in Turkey.