Enhancement of biocompatibility and carbohydrate absorption control potential of rosmarinic acid through crossinking into microparticles

ŞAHİNER M., Blake D. A., Fullerton M. L., SUNER S. C., SUNOL A. K., ŞAHİNER N.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, vol.137, pp.836-843, 2019 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 137
  • Publication Date: 2019
  • Doi Number: 10.1016/j.ijbiomac.2019.07.032
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.836-843
  • Keywords: Rosmarinic acid particles, Antioxidant microgel/nanogel, Inhibition of alpha-glucosidase activity, ANTIDIABETIC DRUGS, ANTIOXIDANT, NANOPARTICLES, ADSORPTION, RELEASE, EXTRACT, GUM
  • Çanakkale Onsekiz Mart University Affiliated: Yes


Rosmarinic acid (RA), a bioflavonoid and antioxidant that exists in plants of the Lamiaceae family, was crosslinked into particles as poly(Rosmarinic Acid) (p(RA)) via an emulsion crosslinking method. The particles were characterized using scanning electron microscopy, Fourier transform infrared spectroscopy, solid state nuclear magnetic resonance C-13 NMR spectroscopy, and thermal gravimetric analysis. The zeta potential values of p(RA) particles were determined at different pHs; the isoelectric point was estimated as pH 1.2. The release of monomeric RA from the particles at 37.5 degrees C was found to be similar at different pHs, 1.0, 7.4, and 11.0. The effects of p(RA) on hemolysis and coagulation were found to be minimal. The antioxidant activity of p(RA) particles and RA monomer were almost indistinguishable suggesting that p(RA) particles may be used as an antioxidant. On a per weight basis, p(RA) particles were similar to 66% less cytotoxic to mammalian cells that RA monomer, as assessed using COS-1 cells. In addition, p(RA) was an 8.6-fold stronger inhibitor of alpha-glycosidase than RA; the IC(50)s of the monomer and particles were 0.121 and 0.014 mg/mL, respectively. The strong inhibitory effect of p(RA) on alpha-glycosidase, coupled with its reduced cytotoxicity and antioxidant activity, provide new opportunities for the use of p(RA). (C) 2019 Elsevier B.V. All rights reserved.